Anti-CD55 / Decay Accelerating Factor (DAF) Monoclonal Antibody(Clone: F4-29D9)
Fig. 1: Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Green). Counterstain is RedDot.
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| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | CD55 |
| Gene ID : | 1604 |
| Uniprot ID : | P08174 |
| Alternative Name : | CD55; CD55 Cromer blood group system; Complement decay-accelerating factor; CROM; DAF; Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF |
| Immunogen Information : | Human umbilical vein endothelial cells (HUVEC) |
Recognizes a single chain glycoprotein of 70kDa, identified as CD55. This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throµghout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml);
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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