Anti-CD54 / ICAM-1 Monoclonal Antibody (Clone:1H4)-Azide free

Product code: 30-1018

Clone name : 1H4
Clonality : Monoclonal
Application : FACS, WB, IHC, IHC-Fr, ICC
Reactivity : Human

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0.1 mg
$389.00 

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Format : Azide free
Amount : 0.1 mg
Isotype : Mouse IgG2b
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : ICAM1
Gene ID : 3383
Uniprot ID : P05362
Alternative Name : ICAM1
Immunogen Information : Raji cells and spleen cells fused with NS1 cells
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Flow Cytometry Recommended dilution: 1 µg/ml
Western Blotting Immunohistochemistry Recommended dilution: 10 µg/ml
Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required Immunohistochemistry (frozen sections) Recommended dilution: 5-10 µg/ml
Immunocytochemistry

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Membrane
Post transnational modification: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
BioGrid: 109610. 178 interactions.
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