Anti-CD43 / Leukosialin Monoclonal Antibody (Clone:MEM-59)-Low Endotoxin
Figure 1: Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59). Lane 1: lysate of human PBL. Lane 2: lysate of canine PBL. Lane 3: lysate of porcine PBL
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| Format : | Low Endotoxin |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
Functional application: The antibody MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells. <br>Flow cytometry: Recommended dilution: 1-5 μg/ml. <br>Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil; recommended dilution: 10 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Nucleus, Nucleus |
| Post transnational modification: | CD43 cytoplasmic tail: Sumoylated. |
| Tissue Specificity: | Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. |
| BioGrid: | 112571. 13 interactions. |
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