Anti-CD4 Monoclonal Antibody (Clone:GK1.5)-Low Endotoxin

CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

Flow Cytometry Recommended dilution: 1 µg/million cells
Immunoprecipitation Recommended dilution: 1-2 µg/100-500 µg of protein in 1 ml lysate
Immunohistochemistry (frozen sections) Immunocytochemistry Recommended dilution: 1-4 µg/ml
Functional Application Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane
Post transnational modification:	Phosphorylated by PKC; phosphorylation plays an important role for CD4 internalization.
Tissue Specificity:	Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.
BioGrid:	198599. 1 interactions.

There are currently no product reviews Write a review on this product!