Anti-CD38 Monoclonal Antibody (Clone:HIT2) Alexa Fluor® 700
Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD38 (HIT2) Alexa Fluor® 700 antibody
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| Format : | Purified |
| Amount : | 100 tests |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.
Flow Cytometry Western Blotting Recommended dilution: 2 µg/ml
Positive control: RAJI human cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Boil for 5 min.
Immunohistochemistry Recommended dilution:10 µg/ml
Immunohistochemistry (frozen sections)
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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