Anti-CD326 / EpCAM Monoclonal Antibody (Clone:323/A3)

Product code: 30-1406

Clone name : 323/A3
Clonality : Monoclonal
Application : FACS
Reactivity : Human

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0.1 mg
$389.00 

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : EPCAM
Gene ID : 4072
Uniprot ID : P16422
Alternative Name : EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Immunogen Information : Human breast cancer MCF-7 cells

CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.

Western blotting: Non-reducing conditions; recommended dilution: 1-2 μg/ml; positive control: MCF-7 cells.
Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 ml lysate.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0); positive tissue: breast carcinoma.
Immunocytochemistry: Recommended dilution: 1-10 μg/ml.
Flow cytometry: Recommended dilution: 1-4 µg/ml.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Lateral cell membrane, Cell junction
Post transnational modification: Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
BioGrid: 110250. 5 interactions.
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