Anti-CD3 zeta (Phospho-Tyr142) Monoclonal Antibody (Clone:EM-54)

Name: Anti-CD3 zeta (Phospho-Tyr142) Monoclonal Antibody (Clone:EM-54)
Brand: Abeomics
SKU: 30-1527
Price: 389.0000 USD
Availability: InStock
Rating: 4.3 (10 reviews)

Figure 1: Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5

Anti-CD3 zeta (Phospho-Tyr142) Monoclonal Antibody (Clone:EM-54)

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Product code: 30-1527

Clone name :	EM-54
Clonality :	Monoclonal
Application :	FACS, WB
Reactivity :	Human, Mouse

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	0.1 mg
Isotype :	Mouse IgG1
Purification :	Purified by protein-A affinity chromatography
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	CD247
Gene ID :	919
Uniprot ID :	P20963
Alternative Name :	CD247, CD3Z, T3Z, TCRZ
Immunogen Information :	CP1 (pTyr142) - KLH

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Subcellular location:	Membrane
Post transnational modification:	Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Tissue Specificity:	CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641).
BioGrid:	107357. 29 interactions.