Anti-CD22 Monoclonal Antibody (Clone:MEM-01)

Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Anti-CD22 Monoclonal Antibody (Clone:MEM-01)

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Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Figure 2: Separation of human CD22 positive lymphocytes (red-filled) from human CD22 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD22 (MEM-01) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Figure 3: Anti-Hu CD22 Purified (clone MEM-01) reactivity pattern in WB application under non-reducing conditions.
The reactivity of MEM-01 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100°C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-01 (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).

CD22 was detected at ~125 kDa in Raji and Daudi cell line.

Product code: 30-1166

Clone name :	MEM-01
Clonality :	Monoclonal
Application :	FACS
Reactivity :	Human

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	0.1 mg
Isotype :	Mouse IgG1
Purification :	Purified by protein-A affinity chromatography
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	CD22
Gene ID :	933
Uniprot ID :	P20273
Alternative Name :	CD22, SIGLEC2
Immunogen Information :	Raji Burkitt's lymphoma cell line

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Flow cytometry: Recommended dilution: 2-4 μg/ml.<br>Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions. The antibody MEM-01 stains only the higher Mw isoform (140 kDa, migrating sometimes as a 125 kDa band) and not the lower Mw isoform (130 kDa, migrating sometimes as a 115 kDa band).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane
Post transnational modification:	Phosphorylated on tyrosine residues by LYN.
Tissue Specificity:	B-lymphocytes.
BioGrid:	107371. 15 interactions.