Anti-CD18 / Integrin beta2 subunit Monoclonal Antibody (Clone:MEM-148)-Low Endotoxin

Figure 1: Western Blotting analysis of PMA-activated neutrophils, using anti-human CD18 (MEM-148). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

Anti-CD18 / Integrin beta2 subunit Monoclonal Antibody (Clone:MEM-148)-Low Endotoxin

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Figure 1: Western Blotting analysis of PMA-activated neutrophils, using anti-human CD18 (MEM-148).
The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

Figure 2: Western Blotting analysis of PMA-activated monocytes, using anti-human CD18 (MEM-148).
The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

Product code: 30-1606

Clone name :	MEM-148
Clonality :	Monoclonal
Application :	FACS
Reactivity :	Human

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Download TDS MSDS

Format :	Low Endotoxin
Amount :	0.1 mg
Isotype :	Mouse IgG1
Purification :	Purified by protein-A affinity chromatography
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	ITGB2
Gene ID :	3689
Uniprot ID :	P05107
Alternative Name :	ITGB2, CD18, MFI7
Immunogen Information :	Peripheral blood mononuclear cells

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Functional application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. <br>Flow cytometry: Recommended dilution: 1-5 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. <br>Western blotting: Non-reducing conditions.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Membrane raft
Post transnational modification:	Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).
Tissue Specificity:	Leukocytes (PubMed:23775590). Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980).
BioGrid:	109895. 36 interactions.