Anti-CD18 / Integrin beta2 subunit Monoclonal Antibody (Clone:MEM-148)-Low Endotoxin
Figure 1: Western Blotting analysis of PMA-activated neutrophils, using anti-human CD18 (MEM-148). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).
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| Format : | Low Endotoxin |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Functional application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. <br>Flow cytometry: Recommended dilution: 1-5 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. <br>Western blotting: Non-reducing conditions.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane, Membrane raft |
| Post transnational modification: | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
| Tissue Specificity: | Leukocytes (PubMed:23775590). Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980). |
| BioGrid: | 109895. 36 interactions. |
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