Anti-CD16 / FcgammaRIII Monoclonal Antibody (Clone:MEM-154)-Low Endotoxin
Figure 1: Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD16 (MEM-154) purified antibody (low endotoxin, concentration in sample 2 μg/ml) GAM APC.
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| Format : | Low Endotoxin |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified by protein-A affinity chromatography |
| Storage condition : | Store at 2-8°C. Do not freeze. |
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms - FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Flow Cytometry Recommended dilution: 5-10 µg/ml
Positive control: PBL (peripheral blood lymphocytes)
Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Functional Application The antibody MEM-154 blocks binding of human IgG to FcgammaRIII.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cell membrane, Secreted |
| Post transnational modification: | The soluble form is produced by a proteolytic cleavage. |
| Tissue Specificity: | Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. |
| BioGrid: | 108508. 11 interactions. |
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