Anti-CD16 / FcgammaRIII Monoclonal Antibody (Clone:MEM-154)-Low Endotoxin

Figure 1: Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD16 (MEM-154) purified antibody (low endotoxin, concentration in sample 2 Î¼g/ml) GAM APC.

Anti-CD16 / FcgammaRIII Monoclonal Antibody (Clone:MEM-154)-Low Endotoxin

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Figure 1: Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD16 (MEM-154) purified antibody (low endotoxin, concentration in sample 2 Î¼g/ml) GAM APC.

Figure 2: Separation of human neutrophil granulocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (MEM-154) purified antibody (low endotoxin, concentration in sample 2 Î¼g/ml) GAM APC.

Product code: 30-1598

Clone name :	MEM-154
Clonality :	Monoclonal
Application :	FACS
Reactivity :	Human

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Low Endotoxin
Amount :	0.1 mg
Isotype :	Mouse IgG1
Purification :	Purified by protein-A affinity chromatography
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	FCGR3A
Gene ID :	2214
Uniprot ID :	P08637
Alternative Name :	FCGR3A, CD16A, FCG3, FCGR3, IGFR3
Immunogen Information :	Human granulocytes

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms - FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Flow Cytometry Recommended dilution: 5-10 µg/ml
Positive control: PBL (peripheral blood lymphocytes)
Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Functional Application The antibody MEM-154 blocks binding of human IgG to FcgammaRIII.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Secreted
Post transnational modification:	The soluble form is produced by a proteolytic cleavage.
Tissue Specificity:	Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
BioGrid:	108508. 11 interactions.