Anti-CD14 / LPS-receptor Monoclonal Antibody (Clone:B-A8)

CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.

ELISA The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 . Immunohistochemistry Recommended dilution: 2-10 µg/ml
Positive tissue: PML brain sections
Application note:
Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels.
Heat retrieval of antigen is recommeded. Flow Cytometry Recommended dilution:1 µg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Secreted, Membrane raft, Golgi apparatus
Post transnational modification:	N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Tissue Specificity:	Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
BioGrid:	107367. 35 interactions.

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