Anti-CD11b / Mac-1 alpha Monoclonal Antibody (Clone:MEM-174)

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Flow Cytometry Recommended dilution:1 µg/ml
Positive control: Peripheral Blood Lymphocytes
Immunoprecipitation Positive control: Granulocytes
CD11b/CD18 transfectants
Application note: Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads)
It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell membrane, Membrane raft
Tissue Specificity:	Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).
BioGrid:	109890. 10 interactions.

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