Anti-Catenin, beta (CTNNB1) Recombinant Rabbit Monoclonal Antibody (Clone:CTNNB1/2030R)
Figure 1: Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Recombinant Rabbit Monoclonal Antibody (CTNNB1/2030R).
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| Format : | Purified |
| Amount : | 100 µg |
| Isotype : | Rabbit IgG, kappa |
| Purification : | Protein A/G |
| Content : | 200µg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throµghout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml);,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Nucleus, Cytoplasm, Cell junction, Cell junction, Cell membrane, Cytoplasm, Cytoplasm, Cell junction, Cytoplasm |
| Post transnational modification: | Deacetylated at Lys-49 by SIRT1. |
| Tissue Specificity: | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (PubMed:29367600). |
| BioGrid: | 107880. 630 interactions. |
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