Anti-c-Myc tag Purified

The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Specificity :The antibody 9E10 can be used to detect the c-Myc tag.

Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 µg/ml; positive tissue: perfused brain sections, liver, spleen.
Immunoprecipitation: Recommended dilution: 1-5 µg/ml; this antibody is not suitable for immunoprecipitation of native c-Myc protein.
Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 µg/ml.
Western blotting: Recommended dilution: 0,5-2 µg/ml; positive control: c-Myc tagged protein.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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