Anti-BOB.1 (B-Cell Marker) Monoclonal Antibody(Clone: BOB1/2422)
Fig. 1: Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2422).
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| Amount : | 100 µg |
| Isotype : | Mouse IgG2b, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | POU2AF1 |
| Gene ID : | 5450 |
| Uniprot ID : | Q16633 |
| Alternative Name : | B cell Oct binding protein 1; B-cell-specific coactivator OBF-1; OCAB; POU domain class 2-associating factor 1 |
| Immunogen Information : | Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary) |
BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has sµggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Western blot 0.5ug/ml- 1ug/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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