Anti-Biotin Mab(1D4-C5)
Figure 1: ELISA analysis of a biotinylated antigen using biotin-specific antibody and streptavidin. Microtitration wells were coated with biotinylated human IgG (0.01 μg/ml) and blocked. Then either anti-biotin mouse monoclonal 1D4-C5 (A, C) or streptavidin-HRP (B) was added in six different doses. Finally, goat anti-mouse-HRP conjugate (A) or streptavidin-HRP (C) was added. HRP signal increased in a dose-dependent manner, but with different dynamics when the biotinylated antibody was detected by anti-biotin mAb/goat anti-mouse-HRP (A) or by streptavidin-HRP (B), and it was falling to zero in a dose-dependent manner when streptavidin-HRP was competed by anti-biotin mAb 1D4-C5 (C).
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Mouse IgG2a kappa |
| Purification : | Purified by protein-A affinity chromatography. |
| Content : | Concentration: 1 mg/ml Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
| Alternative Name : | vitamin H, vitamin B7 |
| Immunogen Information : | biotinylated limulus hemocyanin |
Specificity: Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.
FC (QC tested), ELISA, IP, MC (CyTOF)
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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