Anti-alpha-Tubulin Monoclonal Antibody (Clone: YOL1/34)
Figure 1: Western blotting analysis of human alpha-tubulin using rat monoclonal antibody YOL1/34 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rat anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated anti-rat secondary antibody. A specific band was detected for alpha-tubulin at approximately 54 kDa.
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Rat IgG2a |
| Purification : | Purified by protein-G affinity chromatography. |
| Content : | 1mg/ml, Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
The rat monoclonal antibody YOL1/34 recognizes an epitope of alpha-tubulin localized between amino acids 414-422. It has higher affinity for fixed microtubules than for native ones.
Immunocytochemistry: Recommended dilution 4-8 μg/ml.
Western blotting: Recommended dilution 1-2 μg/ml, reducing conditions.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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