Animal-Free Recombinant Human TWEAK(Discontinued)

Source:E.coli
TWEAK belongs to the TNF family of ligands, and signals through TWEAKR, also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NF-kB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-Gamma. TWEAK also promotes proliferation and migration of endothelial cells. The human TWEAK gene encodes for a 249 amino acid type II transmembrane protein, which contains a 21 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 207 amino acid extracellular domain. Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein.

Assay #1:  The ED₅₀ as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.  Assay #2:  TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-Gamma.  The ED₅₀ for this effect is between 30-45 ng/ml.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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