Alexa Fluor 488 Conjugated Anti-Human IL-2 MAb (Clone: 35C3)
Figure 1: Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Alexa Fluor 488 antibody (10 μl reagent / 100 μl of peripheral whole blood).
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| Amount : | 100 tests |
| Isotype : | Mouse IgG2b kappa |
| Purification : | Purified antibody is conjugated with Alexa Fluor 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography |
| Content : | Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.
Specificity: The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular).
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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